Advance Bioanalytical Research With Us
Customized Bioanalytical Solutions
Northern Bio offers customized bioanalytical assays for molecular biology services and ligand binding assays for immunogenicity and biomarker analysis to support all phases of therapeutic development. Collaborating scientist-to-scientist, Northern Bio develops tailored solutions to accelerate the advancement of next-generation therapeutics.
Consult a Scientist
We are a team of scientists who value open communication and collaboration. We work closely with our sponsors to provide customized solutions and expert support.
Advanced Molecular Biology for Drug Development
Capabilities and Instrumentation
qPCR
Amplifies and quantifies specific DNA sequences in real-time using fluorescent dyes or probes to monitor the amplification process.
RT-qPCR
Quantifies RNA levels by reverse transcribing RNA to cDNA, followed by real-time qPCR amplification and detection.
dPCR
Partitions DNA samples into thousands of individual reactions to provide absolute quantification of target nucleic acids without relying on standard curves.
Stem-loop RT-qPCR for miRNA
Uses a stem-loop primer to reverse transcribe microRNAs (miRNAs) into cDNA, followed by qPCR for sensitive and specific quantification of small non-coding RNAs.
Methods
Methods are used for quantification of cell and gene therapeutics such as viral vector, LNP mRNA, small RNA (e.g. miRNA, siRNA), and cells (e.g., stem cell, CAR-T cells).
Instrumentation
QuantStudio 7 Flex Real-Time PCR Systems
QIAcuity One & Four - 5plex system for digital PCR
QIASymphony for automated DNA/RNA extraction
Nanodrop Spectrophotometer 8000
QIAgility Liquid Handling Robot
Precellys Tissue Homogenizer
Agilent Bioanalyzer
Azure 200 Imaging System
Method Development Timelines
Our expert team specializes in developing and validating advanced PCR-based assays, delivering customized solutions to meet your research needs. Consulting with one of our experienced study directors, a tailored timeline for method development can be proposed, accounting for assay complexity, analyte characteristics, and project requirements to ensure flexibility and precision. With a steadfast commitment to regulatory compliance and scientific rigor, we craft high-performance assays to deliver reliable results, advancing your research with efficiency and accuracy.
Immunoassays for Nonclinical Research
The Northern Bio team excels in immunoassay development and validation across a wide range of methodologies in support of nonclinical programs. We utilize several platforms to provide customizable solutions for immunoassay development and biomarker testing.
Capabilities and Instrumentation
Anti-Drug Antibody (ADA) assays for capsid or protein
Detect and quantify antibodies generated against therapeutic proteins or viral capsids, assessing immunogenicity in drug development.
Biomarker analysis - Neurofilament Light Chain
Assay measures neurofilament levels in biofluids, serving as a biomarker for neuronal damage in neurodegenerative diseases.
Biomarker analysis - Cytokine Multiplex Analysis
Simultaneously quantifies multiple cytokines in a sample, enabling comprehensive immune response profiling.
Biomarker analysis - Complement Analysis
Evaluates the activity or levels of complement system proteins, aiding in the study of immune-related disorders or therapeutic effects.
Custom ligand binding PK assays
Detect unique analytes in a variety of biofluids and tissues.
Instrumentation
Meso Sector 600MM
Spectramax iD5
Luminex 200
Frequently Asked Questions (FAQs) about Bioanalytical Services
What sort of assays does Northern Bio support?
Northern Bio routinely develops customized assays for molecular biology (qPCR, RT-PCR, dPCR, Stem-loop RT-qPCR) and immunogenicity (ADA, Nf-L, cytokine, complement, etc.).
What instrumentation does Northern Bio utilize to support assay development and analysis?
Northern Bio operates on the most advanced technologies for bioanalytical support. For our molecular biology assays, Northern Bio has been equipped with QuantStudio 7, QIAcuity, QIASymphony, and Nanodrop Spectrophotometer platforms. Immunoassay development and analysis, Northern Bio works on Meso Sector 600MM, Spectramax iD5, and Luminex systems.
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